After conceding twice in the opening 30 minutes, it was an uphill battle for the home side, with UWA suffering only their second home loss of the season at the hands of Western Knights.
It was horrid start for the home side, with a misscommunication at between the defence and Ranjeet Singh allowing Adriano Jelenovic the easiest of finishes with only five minutes on the clock.
The gift of a goal gave the State League Cup champions confidence, with Uni struggling to gain a foothold in the contest.
Knights then doubled their lead through Jelenovic again, with the defence unable to clear a dangerous corner and the striker made no mistake from six-yards after the ball kindly fell at his feet.
After a nervy opening half hour, UWA began to play some of their trademark open attacking football.
At the 41st minute, a superb ball through from Luke Randazzo had striker Alex Kretowicz through on goal, but his strike was well blocked.
It was much of the same in the second stanza, with Uni pressing for an equaliser, but lacking composure with the final ball.
The derby ended 2-0 to the visitors, with the trip to Bunbury next week against Phoenix now shaping up to be another massive encounter.
Skipper Josh Marocchi lamented the early lapses in concentration at the back as he reflected on a missed opportunity for Uni.
"Unfortunately, we were not able to settle into our rhythm and allowed Western Knights to dominate the proceedings early on in the piece," he said.
"We had a better spell in the second half and overall limited Knights to minimal shots on goal but we were unable to create any clear cut chances ourselves to put Western Knights on the back foot,"
"Credit must go to our opposition for limiting our attacking threats on the day," the captain added.
After the match the centre-back implored the squad to dig deep over the final month of the campaign despite the setback.
"As a team, we must learn from this game and rededicate our efforts over the next four weeks."
There is still much to play for this season and we need to be prepared for the challenges that await us week in week out starting with Phoenix this weekend. I know we will be stronger and more focused as a result on last Saturday," he added.