The Uni boys have claimed bragging rights in the Western Suburbs Derby in Round 8 following a 2-0 win at The Mac.
In what was a typically tense and physical encounter between the two sides, Tim Robertson's powerful header on the hour mark and Lloyd Prout's sealer in the 86th-minute saw UWA claim a deserved three points.
The win continued the side's recent resurgence as they climb away from the relegation zone and within a win of the leading pack.
“We kept our shape well today and cut down their space, and we wanted to see if they had a Plan B, today they didn’t," Michael Janssen said.
“Our goals were well created and taken and the players will enjoy this – any derby win is special.”
The hosts started the match brightly, with Alex Kretowicz forcing Knights goalkeeper Sam Stone into a couple of smart saves early doors before going desperately close to opening the scoring with his lob clipping the crossbar and over with 15 minutes played.
Knights fought their way into the contest with their set-pieces causing a few issues for the UWA defence, but they were able to hold firm as 'keeper Alex Preece enjoyed a superb day between the sticks.
The second-half saw the chances come more frequently, with Kretowicz and Rycroft coming close for the respective sides in the opening stages.
Uni then took the lead in the 58th-minute as Robertson rose above the Knights defence to head home from Bryce Conway's whipped corner, much to the delight of the home faithful.
Janssen's side showed discipline and determination to hold their shape and force Knights into a series of long balls as they searched for an equaliser. They came close through Mao Loko who hit the underside of the crossbar from a Rycroft cross, but it was UWA who would double their lead late on.
Skipper Josh Marocchi's clever chipped ball found Conway in space down the left-wing, with the substitute able to find Conor Devlin as the central midfielder then delivered an inch-perfect ball to the on-rushing Prout who duly dispatched into the back of the net to seal a massive win for the club.
In other results, the 18's claimed a massive 14-1 win as they remain clear at the top of the league, while the reserves succumbed to a classy Knights outfit losing 2-0.